怀山药皮对淇河鲫血清生化、免疫指标及组织结构的影响

为探究怀山药皮(Chinese yam peel,YP)对淇河鲫免疫的影响,选取平均体重为(106.76±3.74)g的淇河鲫240尾,随机分为对照组(NC)、0.5%怀山药皮添加组(LYP)、1%怀山药皮添加组(MYP)、2%怀山药皮添加组(HYP)4组,养殖8周,检测实验鱼生长指标、血清生化指标及免疫相关基因的表达情况。结果显示:添加怀山药皮对淇河鲫生长指标没有显著性影响;血液生化指标中谷草转氨酶活性在MYP组显著性降低,碱性磷酸酶的活性显著升高;与NC组相比,怀山药皮添加组肝脏细胞膜与核膜完整性增强,胞质破碎,核溶解、偏移现象减轻;与NC组相比,添加怀山药皮上调了抑炎因子(IL-10)、紧密连接蛋白基因(Claudin-1)、抗氧化基因(CAT和GST)和TLR通路基因(TLR4)mRNA表达量。以上结果表明,饲料中添加怀山药皮,能在一定程度上增强鱼体免疫力。     

热风干燥过程中山药水分状态的变化研究

为了解山药的水分赋存状态的变化，以襄阳道地山药为材料，分别在60、70、80、90、100 ℃条件下进行热风干燥，采用低场核磁共振（LF-NMR）和差式量热扫描（DSC）技术，每隔15 min测定山药在热风干燥过程中的水分状态及迁移规律。结果表明，在60~90 ℃条件下，温度越高，干基含水率的降速越快。山药的T2弛豫图谱有3个较为明显的吸收峰，随干燥进程的延续，各峰面积均明显减少，其中自由水所在峰的面积降幅最大，表明干燥过程中自由水散失最多，而且自由水逐渐向半结合水和结合水迁移，冷冻峰和解冻峰也随之变小。但在100 ℃下干燥时，样品可能因表面板结导致干基含水率、低场核磁吸收峰升高，冷冻和解冻峰面积增加。因此，在实际干燥过程中，山药的热风干燥温度不宜高至100 ℃。     

Effects of elevated CO2 concentration on bulbil germination and early seedling growth in Chinese yam under different air temperatures

The present study investigated the effects of elevated carbon dioxide concentration ([CO₂]) and air temperature on the germination of seed bulbils and the seedling vigour of two Chinese yam lines. Plants were grown under two [CO₂] levels, ambient and elevated (ambient + 200 μmol mol^?1), and two mean air temperature regimes, 22.2 °C (ambient + 1.4 °C) and 25.6 °C (ambient + 5.2 °C). Elevated [CO₂] did not affect bulbil germination under both air temperature regimes. During the early growth stage, the dry weight (DW) of leaves, vines, shoots, roots, belowground parts (roots + tubers) and whole plants were higher under elevated [CO₂] than ambient [CO₂] for both lines under the low- and high-temperature regimes. The values of vigour indexes (index I = germination % × seedling length and index II = germination % × seedling DW) were also higher under elevated [CO₂] than ambient [CO₂] for both lines. These results indicated that Chinese yam seedlings respond positively to elevated [CO₂] during the early growth stage. The above:belowground DW ratios were lower under elevated [CO₂] than ambient [CO₂] in seedlings with very small new tubers for both yam lines, indicating that elevated [CO₂] strongly affected the root growth in the early growth stage. The DWs of post-treatment seed bulbils were higher in the elevated [CO₂] under both air temperature regimes. The results showed that Chinese yam used a smaller amount of the reserves in seed bulbils under elevated [CO₂] than under ambient [CO₂].     

利用CRISPR/Ca9技术靶向编辑芥蓝BoaZDS

以芥蓝(Brassicaoleraceavar.alboglabra)为材料,以ζ–胡萝卜素脱氢酶(ζ-Carotene desaturase,ZDS)基因为目标基因,建立其CRISPR/Cas9基因组编辑体系。在BoaZDS的编码区近5′端选择靶位点,构建了CRISPR/Cas9表达载体,通过农杆菌介导的遗传转化方法获得了19个芥蓝转基因阳性植株,Sanger测序分析发现其中13株成功突变,CRISPR/Cas9载体在芥蓝上的突变效率为68.42%,且所有突变植株均表现出明显的白化表型。     

中草药与苯扎溴铵联用治疗车轮虫病的案例分析

车轮虫病是鱼类重要的常见纤毛虫病。近年来,对传统化学药物耐药的车轮虫和小车轮虫越来越多。本文介绍了复方中草药联合苯扎溴铵治疗河鲀、黄颡鱼和草鱼车轮虫病的典型案例,以期为类似病例治疗提供参考。     

Forest recovery after clear-cutting in Chinese pine(Pinus tabuliformis) plantations of North China

In Hebei Province of North China, forest was recovered with natural recruitment in plantations with large area of clear-cutting Chinese pine(Pinus tabuliformis). This study was aimed to demonstrate the dynamic characteristics of recruits during the natural recruitment. Both plot survey and the spatial point-pattern analysis were performed. Five developmental stages of natural recruitment were selected and studied, including 1 year before and 2, 5, 8, and 11 years after clear-cutting. Different slope aspects were also included. Natural recruitment was always dominated by Chinese pine with a proportion of higher than 90%. For plots of 1 year before clear-cutting on east-and north-facing slopes, recruit densities were 7886 and 5036 stems/hm2, the average heights were 0.78(±0.85) and 1.06(±1.15) m, and the average diameters at breast height(DBH) were 3.21(±1.38) and 2.91(±1.38) cm, respectively. After clear-cutting, recruit density was initially increased, then it was gradually declined with time; however, the variation of average DBH was contrary to that of recruit density. Both of them were no longer varied between 8 and 11 years after clear-cutting. The average height of recruits continued to increase after clear-cutting. For the plots of 11 years after clear-cutting on east-and north-facing slopes, average heights of recruits reached 2.00(±1.14) and 2.24(±1.20) m, respectively. The statuses of recruits on north-facing slopes were better than those on east-facing slopes after clear-cutting. Meanwhile, recruits on east-facing slopes were always aggregated at small scales, while spatial pattern of recruits varied with time on north-facing slopes. Moreover, forest was recovered more quickly by natural recruitment than by artificial afforestation after clear-cutting. The structural diversity was higher in naturally regenerated forests than in plantations of the same age. Our results demonstrated that clear-cutting of Chinese pine plantations recovered by natural recruitment has the potential to be an effective approach for establishing multifunctional forest.     

复合涂膜保鲜剂对番荔枝模拟贮运期间品质的影响

研究大黄与五倍子复合涂膜保鲜剂涂膜对番荔枝模拟贮运期间贮藏品质的影响，明确该保鲜剂的保鲜效果。将保鲜剂涂膜的番荔枝作为处理组、未涂膜的果实作为对照(CK)，分别置于加冰的泡沫箱中贮藏20 h模拟当地贮运技术，之后开箱放置于常温下贮藏，考察复合涂膜保鲜剂对番荔枝感官品质及其生理生化的影响。结果表明，与CK组比较，经涂膜处理后番荔枝果实的裂果、烂果、霉果和失重情况明显改善,褐变指数和乙烯释放量显著降低；有效延缓了果实软化，延迟了呼吸高峰第1次出现的时间，抑制呼吸高峰的第2次出现， 可显著降低淀粉酶活性、减缓淀粉转化为可溶性固形物的速度。该保鲜剂可有效延缓番荔枝模拟贮运期间果实的衰老，起到较为理想的保鲜效果。     

靖州杨梅引种试验与表型聚类分析

以靖州的传统品种木洞光叶和木洞大叶杨梅为对照,比较引进的早佳、早荠蜜梅、晚荠蜜梅、黑晶、荸荠种、东魁6个品种的叶片形态、果实品质、成熟期等主要农艺性状,并对品种叶片和果实表型性状进行聚类分析。结果表明,早佳与木洞光叶杨梅聚为一类,但早佳成熟期提早约7 d,表现出较好的早熟特性,具有极好的推广应用前景;荸荠种具有核小、可食率高等特点,在靖州表现出良好的适应性;东魁果大、商品性佳,其株产和售价是当地传统品种的1.96~1.98倍和2.67~3.48倍,栽培经济效益显著。